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KMID : 1134819970260030534
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Journal of the Korean Society of Food Science and Nutrition
1997 Volume.26 No. 3 p.534 ~ p.539
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Immobilization of Fructosyltransferase to a Porous Carrier Bearing Quaternary Alkyl Alkanolammonium Groups
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Chung Mi-Sun
Lee Sun-Hee
Jhon Deok-Young
Hwang Keum-Taek
Uhm Tai-Boong
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Abstract
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In order to reuse enzyme efficiently, a method for ionic binding of fructosyltransferase to a porous carrier bearing quaternary alkyl alkanolammonium groups was investigated. The fructosyltransferase activity of the immobilized enzyme increased with increasing amount of loaded enzyme, and maximally reached 770U/g of the carrier when loaded amount of the enzyme was 18.2㎎/g carrier. The immobilized fructosyltransferase had optimum pH and temperature of 7.5 and 45℃, respectively, whereas soluble enzyme had 6.5 and 55℃ : the Km value for the immobilized enzyme was 27.8 mM for sucrose, which was the same as that of soluble enzyme. In a batch reactor, the enzyme produced a mixture of fructooligosaccharides, mainly F₂G to F_5G, from sucrose with the slight loss of enzyme activity during continuous operation of 12 days at 42℃.
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KEYWORD
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immobilization, fructosyltransferase, fructooligosaccharides
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